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Rodent anesthesia and analgesia: Theory and practical use of anesthetic machine


2024-03-29 - 2024-03-29 ( 6 hours )


Διά ζώσης

Υπεύθυνος Προγράμματος:
Anastasia Tsingotjidou
Η υποβολή αιτήσεων ολοκληρώνεται στις

Rodent anesthesia and analgesia: Theory and practical use of anesthetic machine

Η υποβολή αιτήσεων ολοκληρώνεται στις



  • Σύντομη Περιγραφή

    The Center for Education and Lifelong Learning of the Aristotle University of Thessaloniki welcomes you to the “Rodent anesthesia and analgesia: theory and practical use of anesthetic machine” workshop, six hours in person course at the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, AUTh.

    The program will present current approaches on rodent anesthesia and analgesia, and exhibit use of an anesthetic machine. Understanding rodent anesthesia and analgesia is crucial for ensuring humane and ethical treatment of animals in research practice, and a requirement from the European legislation protecting the animals used in research. Participants will receive a Certificate (1 ECTS) following full attendance and a short examination.

    The Scientific Director of the Program is Anastasia Tsingotjidou, Assistant Professor in Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences. She has vast experience in the use of laboratory animals in research, is the President of the National Committee for the Welfare of Animals used for Scientific Purposes, and the Chair of the Organizing Committee for the FELASA 2025 meeting:

    Start Date: Friday, 29 March 2024

    End of the course: Friday, 29 March 2024

    Duration: 6 hours

    Cost: 200 euros

    Discount: not applicable

    Certificate/ECTS: Yes/1

    Applications are submitted online from 20/03/2024 to 28/03/2024


    Teaching Staff

    Pavlos Lelovas, DVM, PhD, Designated Veterinarian, Lab of Experimental and Research Surgery, Medical School, National Kapodistrian University of Athens

    Vasileios Ntafis, DVM, MSc, PhD, Head, Animal Facilities, BSRC ALEXANDER FLEMING

    Cholawat Pacharinsak, Associate Professor at Stanford University’s Department of Comparative Medicine; DVM, PhD, DACVAA:

    Patrick Sharp, DVM, MRCVS, MANZCVS, DACLAM

    Anastasia Tsingotjidou, Assistant Professor in Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences:

    Argyro Zacharioudaki, DVM MLAS Dipl. ECLAM, BIOEMTECH Laboratories

  • Στόχος του προγράμματος

    The aim of the workshop is to provide participants with the knowledge, skills, and confidence needed to effectively and ethically administer anesthesia and analgesia to rodents (mice and rats), thereby promoting the welfare of animals used in research and improving the quality and translatability of scientific outcomes.

  • Μαθησιακά Αποτελέσματα

    Upon completion of the course, participants will be able to identify and select appropriate anesthetic agents and techniques for rodents based on factors such as species, age, weight, and experimental requirements. They should understand the pharmacokinetics, dosages, and routes of administration for commonly used agents. Additionally, they should be able to handle and use an anesthetic machine to provide inhalational anesthesia.

    Participants will be competent in monitoring vital signs during anesthesia, including heart rate, respiratory rate, temperature, and blood pressure, and should understand how to respond to deviations from normal values. They should also learn about supportive measures to maintain physiological stability.

    They should develop problem-solving skills to address challenges encountered during anesthesia and analgesia procedures, including troubleshooting equipment issues, managing complications, and making informed decisions about anesthesia protocols.

  • Σε ποιους απευθύνεται

    The program is addressed to veterinarians, medical doctors, dentists, pharmacists, and all scientists from biological sciences, or from other scientific backgrounds such as psychology and/or engineering, who use rodents for scientific purposes.  Veterinary medicine students in the last year of their studies will also be accepted.

  • Κριτήρια επιλογής

    Prerequisites to submit an Application Form:

    • Access to Internet
    • Personal email
    • Basic computer skills
    • English knowledge

    The above are required to complete the examination following the theory and the practice.

  • Μέθοδος Υλοποίησης

    The program contains theoretical and practical presentation of:

    • inhalational and injectable anesthesia techniques for rodents
    • anesthetic machine and its components
    • complete anesthesia set-up, perioperative monitoring equipment, and routine system maintenance
    • practical analgesia formulations and techniques for rodents
    • “Green Vivarium” solutions

    During the discussion session, participants are encouraged to bring questions and information for other species used at their facilities.

  • Πρόγραμμα Σπουδών

    Workshop Program

    9.30 – 10.00 Introduction
    10.00 – 10.30 Inhalational anesthesia
    10.30 – 11.00 Injectable Anesthesia
    11.00 – 11.30 Analgesia
    11.30 – 12.00 Green Vivarium
    12.00 – 13.00 Lunch
    13.00 – 14.00 Discussion (Q & A)
    14.00-14.30 Break
    14.30-16.30 Use of anesthetic machine for rodents
    16.30-17.00 Online test


  • Εκπαιδευτικό Υλικό / Παροχές
    1. Navarro, K, Huss, MK, Sharp, P, Smith, J, Marx, J, Pacharinsak, C. Mouse Anesthesia – The Art and Science. ILAR. 2021. 1: 1-30.
    2. Pacharinsak, C. Smith, JC. (Ed) (2017) Handbook of Anesthesia and Pain Management in Laboratory Animals: Rodents, 1st Edition. CRC Press, Taylor and Francis group, Boca Raton,
    3. Discussion with the instructors of the program on other species anesthesia
  • Υποχρεώσεις και Δικαιώματα Εκπαιδευομένων

      Obligations of Trainees:

    1. Participation in the programs of K.E.DI.BI.M. of the AUTH implies full acceptance of the program’s Study Guide and the Internal Regulation of Operation of the K.E.DI.BI.M. of the AUTH.
    2. The presence/attendance of trainees in the educational programmes is mandatory. In the programmes which are realized in physical presence, as in those implemented using the modern method of tele-education, the study is generally mandatory and the limit of absences cannot exceed 10% of the prescribed training hours. The monitoring of asynchronous education is implemented according to the study schedule set by each programme.
    3. For the successful completion of the programme, the participants should:
    4. not exceed 10% absences of the scheduled training hours
    5. have passed the exams
    6. have paid all the tuition fees by 28/03/2024

    Rights of Trainees:

    1. Trainees are informed by the Department of Administrative Support of K.E.DI.BI.M. for all kinds of information related to the operation of the Centre.
    2. Trainees are supported electronically through the educational platform by the trainers, as part of the educational process, to resolve questions and provide clarifications related to the thematic units of the programme.
    3. Trainees have the right to apply for the interruption/termination of their programme attendance and a refund of their tuition fees, in accordance with the provisions of par. 12.9 hereof.
    4. Students’/Trainees’ work is protected under the Copyright Regulations/Provisions.
  • Πιστοποιητικό

    After successful completion of the program, participants receive a Certificate of Specialised Training on rodent anesthesia and analgesia which is issued by the Center for Education and Lifelong Learning of the AUTH and signed by the President of the Center.

    Participants, who have attended but not successfully completed the program, will be awarded a Certificate of Attendance.

  • Επικοινωνία

    For further information, please contact:

    Name/Surname: Anastasia Tsingotjidou

    Tel: +30 2310999941


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Tuition fees is 200 euros. Final date of deposit is 28/03/2024.

Acount number: 5202-057157-381

IBAN: GR94 0172 2020 0052 0205 7157 381

Code Bic (SWIFT Address): PIRBGRAA

When you submit your deposit please write your name/surname along with the code 10667.

Candidates submit the Application Form online. Each application has to be accompanied by a CV stating the involvement in the use of animals (especially rodents) in research. Supporting documents can be sent to the following email:

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